PDBe 6nd1

Electron Microscopy
4.1Å resolution

CryoEM structure of the Sec Complex from yeast

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Protein transport protein SEC61 Chain: B
Molecule details ›
Chain: B
Length: 480 amino acids
Theoretical weight: 52.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32915 (Residues: 1-480; Coverage: 100%)
Gene names: L3502.5, SEC61, YLR378C
Sequence domains:
Protein translocation protein SEC63 Chain: A
Molecule details ›
Chain: A
Length: 677 amino acids
Theoretical weight: 76.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14906 (Residues: 1-663; Coverage: 100%)
Gene names: NPL1, PTL1, SEC63, YOR254C
Sequence domains: DnaJ domain
Protein transport protein SSS1 Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35179 (Residues: 1-80; Coverage: 100%)
Gene names: D4475, SSS1, YDR086C
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein transport protein SBH1 Chain: D
Molecule details ›
Chain: D
Length: 82 amino acids
Theoretical weight: 8.72 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P52870 (Residues: 1-82; Coverage: 100%)
Gene names: SBH1, SEB1, YER087BC, YER087C-B
Sequence domains: Sec61beta family
Translocation protein SEC66 Chain: E
Molecule details ›
Chain: E
Length: 206 amino acids
Theoretical weight: 24.26 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33754 (Residues: 1-206; Coverage: 100%)
Gene names: HSS1, SEC66, SEC71, YBR1232, YBR171W
Sequence domains: Preprotein translocase subunit Sec66
Translocation protein SEC72 Chain: F
Molecule details ›
Chain: F
Length: 193 amino acids
Theoretical weight: 21.63 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39742 (Residues: 1-193; Coverage: 100%)
Gene names: L8003.18, SEC67, SEC72, SIM2, YLR292C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-0440
Expression system: Saccharomyces cerevisiae