› 6c2h

X-ray diffraction
1.49Å resolution

Crystal Structures of Cystathionine beta-Synthase from Saccharomyces cerevisiae: the Structure of the Catalytic Core

Released:
DOI: 10.2210/pdb6c2h/pdb
PDB entry 6c2h coloured by chain, front view.
PDB entry 6c2h coloured by chain, side view.
PDB entry 6c2h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal Structures of Cystathionine β-Synthase from Saccharomyces cerevisiae: One Enzymatic Step at a Time.
Tu Y, Kreinbring CA, Hill M, Liu C, Petsko GA, McCune CD, Berkowitz DB, Liu D, Ringe D
Biochemistry 57 3134-3145 (2018)
PMID: 29630349

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32582 (Residues: 1-353; Coverage: 70%)
Gene names: CYS4, G6667, STR4, YGR155W
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
7 bound ligands:
Ligand ACT 1 x ACT
Ligand CA 1 x CA
Ligand NA 1 x NA
Ligand CL 1 x CL
Ligand PEG 3 x PEG
Ligand PGE 1 x PGE
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 81.374Å b: 81.374Å c: 208.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.128 0.126 0.162
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.
Zuhra et al. (2020)
2 more

1 mention without citation

Crystal Structures of Cystathionine β-Synthase from Saccharomyces cerevisiae: One Enzymatic Step at a Time.
Tu et al. (2018)