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A conserved interaction of the dynein light intermediate chain with dynein-dynactin effectors necessary for processivity.

Lee IG, Olenick MA, Boczkowska M, Franzini-Armstrong C, Holzbaur ELF, Dominguez R
Nat Commun 9 986 (2018)
Cited: 51 times
EuropePMC logo PMID: 29515126

Abstract

Cytoplasmic dynein is the major minus-end-directed microtubule-based motor in cells. Dynein processivity and cargo selectivity depend on cargo-specific effectors that, while generally unrelated, share the ability to interact with dynein and dynactin to form processive dynein-dynactin-effector complexes. How this is achieved is poorly understood. Here, we identify a conserved region of the dynein Light Intermediate Chain 1 (LIC1) that mediates interactions with unrelated dynein-dynactin effectors. Quantitative binding studies map these interactions to a conserved helix within LIC1 and to N-terminal fragments of Hook1, Hook3, BICD2, and Spindly. A structure of the LIC1 helix bound to the N-terminal Hook domain reveals a conformational change that creates a hydrophobic cleft for binding of the LIC1 helix. The LIC1 helix competitively inhibits processive dynein-dynactin-effector motility in vitro, whereas structure-inspired mutations in this helix impair lysosomal positioning in cells. The results reveal a conserved mechanism of effector interaction with dynein-dynactin necessary for processive motility.

Articles - 6b9h mentioned but not cited (2)

  1. A conserved interaction of the dynein light intermediate chain with dynein-dynactin effectors necessary for processivity. Lee IG, Olenick MA, Boczkowska M, Franzini-Armstrong C, Holzbaur ELF, Dominguez R. Nat Commun 9 986 (2018)
  2. Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. Olenick MA, Dominguez R, Holzbaur ELF. J Cell Biol 218 220-233 (2019)


Reviews citing this publication (11)

  1. The cytoplasmic dynein transport machinery and its many cargoes. Reck-Peterson SL, Redwine WB, Vale RD, Carter AP. Nat Rev Mol Cell Biol 19 382-398 (2018)
  2. Dynein activators and adaptors at a glance. Olenick MA, Holzbaur ELF. J Cell Sci 132 jcs227132 (2019)
  3. Activation and Regulation of Cytoplasmic Dynein. Canty JT, Yildiz A. Trends Biochem Sci 45 440-453 (2020)
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  5. Selective motor activation in organelle transport along axons. Cason SE, Holzbaur ELF. Nat Rev Mol Cell Biol 23 699-714 (2022)
  6. The crosstalk between microtubules, actin and membranes shapes cell division. Rizzelli F, Malabarba MG, Sigismund S, Mapelli M. Open Biol 10 190314 (2020)
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  8. Cargo-Mediated Activation of Cytoplasmic Dynein in vivo. Xiang X, Qiu R. Front Cell Dev Biol 8 598952 (2020)
  9. The Generation of Dynein Networks by Multi-Layered Regulation and Their Implication in Cell Division. Torisawa T, Kimura A. Front Cell Dev Biol 8 22 (2020)
  10. Review: Cytoplasmic dynein motors in photoreceptors. Dahl TM, Baehr W. Mol Vis 27 506-517 (2021)
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Articles citing this publication (38)

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  12. RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Keren-Kaplan T, Sarić A, Ghosh S, Williamson CD, Jia R, Li Y, Bonifacino JS. Nat Commun 13 1506 (2022)
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  17. Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation. Yap CC, Digilio L, McMahon LP, Wang T, Winckler B. J Neurosci 42 4415-4434 (2022)
  18. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS. J Cell Biol 220 e202005184 (2021)
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  31. Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in Toxoplasma. Chan AW, Broncel M, Yifrach E, Haseley NR, Chakladar S, Andree E, Herneisen AL, Shortt E, Treeck M, Lourido S. Elife 12 RP85654 (2023)
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  37. Recombinant dynein light intermediate chain of Haemonchus contortus affects the functions of goat immune cells in vitro. Aimulajiang K, Wen Z, Khand FM, Leghari A, Xu L, Song X, Li X, Yan R. Parasitol Res 121 1699-1707 (2022)
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