PDBe 6hkt

X-ray diffraction
9.7Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 50-mer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H3.1 Chains: A, E, K, O, U, Y, a, e, k, o, u, y
Molecule details ›
Chains: A, E, K, O, U, Y, a, e, k, o, u, y
Length: 139 amino acids
Theoretical weight: 15.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P68431 (Residues: 1-136)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Histone H4 Chains: B, F, L, P, V, Z, b, f, l, p, v, z
Molecule details ›
Chains: B, F, L, P, V, Z, b, f, l, p, v, z
Length: 106 amino acids
Theoretical weight: 11.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P62805 (Residues: 1-103)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Histone H2A type 1-B/E Chains: 0, 2, C, G, M, Q, W, c, g, m, q, w
Molecule details ›
Chains: 0, 2, C, G, M, Q, W, c, g, m, q, w
Length: 133 amino acids
Theoretical weight: 14.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P04908 (Residues: 1-130)
Gene names: H2AFA, H2AFM, HIST1H2AB, HIST1H2AE
Histone H2B type 1-J Chains: 1, 3, D, H, N, R, X, d, h, n, r, x
Molecule details ›
Chains: 1, 3, D, H, N, R, X, d, h, n, r, x
Length: 129 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P06899 (Residues: 1-126)
Gene names: H2BFR, HIST1H2BJ
DNA (1122-MER) Chain: I
Molecule details ›
Chain: I
Length: 1122 nucleotides
Theoretical weight: 344.76 KDa
DNA (1122-MER) Chain: J
Molecule details ›
Chain: J
Length: 1122 nucleotides
Theoretical weight: 348.51 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 111.078Å b: 238.761Å c: 674.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.254 0.286
Expression system: Escherichia coli BL21(DE3)