PDBe 6hbo

Solution NMR

Solution structure of the ASHH2 CW domain with the N-terminal histone H3 tail mimicking peptide monomethylated on lysine 4

Released:
Entry authors: Dobrovolska O, Madeleine N, Halskau O

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase ASHH2 Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 8.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2LAE1 (Residues: 861-928; Coverage: 4%)
Gene names: ASHH2, At1g77300, EFS, LAZ2, SDG8, SET8, T14N5.15
Sequence domains: CW-type Zinc Finger
Histone H3.3 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 2-10; Coverage: 7%)
Gene names: H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 3%
Refinement method: distance geometry
Chemical shifts: BMR27251  
Expression systems:
  • Escherichia coli
  • Not provided