PDBe 6gfi

X-ray diffraction
2.3Å resolution

Structure of Human Mesotrypsin in complex with APPI variant T11V/M17R/I18F/F34V

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PRSS3 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q8N2U3 (Residues: 28-251; Coverage: 89%)
Gene name: PRSS3
Sequence domains: Trypsin
Soluble APP-beta Chains: C, E
Molecule details ›
Chains: C, E
Length: 81 amino acids
Theoretical weight: 9.19 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P05067 (Residues: 294-346; Coverage: 7%)
  • Best match: P05067-7 (Residues: 294-346)
Gene names: A4, AD1, APP

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P31
Unit cell:
a: 53.215Å b: 53.215Å c: 237.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.245 0.301
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Komagataella phaffii GS115