PDBe 6g79

Electron Microscopy
3.78Å resolution

Coupling specificity of heterotrimeric Go to the serotonin 5-HT1B receptor

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the serotonin 5-HT
OpenAccess logo Nature (2018)
PMID: 29925951
Related structures: EMD-4358

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09471 (Residues: 4-57, 182-354; Coverage: 61%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
5-hydroxytryptamine receptor 1B Chain: S
Molecule details ›
Chain: S
Length: 364 amino acids
Theoretical weight: 41.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28222 (Residues: 34-390; Coverage: 92%)
Gene names: HTR1B, HTR1DB
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.78Å
Relevant EMDB volumes: EMD-4358
Expression systems:
  • Trichoplusia ni
  • Escherichia coli