PDBe 6f09

X-ray diffraction
1.59Å resolution

Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide


Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Ubiquitin carboxyl-terminal hydrolase 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 13 amino acids
Theoretical weight: 1.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P40818 (Residues: 712-724; Coverage: 1%)
Gene names: KIAA0055, UBPY, USP8

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 60.379Å b: 64.89Å c: 71.519Å
α: 99.71° β: 114.22° γ: 108.79°
R R work R free
0.182 0.18 0.225
Expression systems:
  • Escherichia coli
  • Not provided