PDBe 6erq

X-ray diffraction
4.5Å resolution

Structure of the human mitochondrial transcription initiation complex at the HSP promoter

Released:
Source organism: Homo sapiens
Primary publication:
Structural Basis of Mitochondrial Transcription Initiation.
Cell 171 1072-1081.e10 (2017)
PMID: 29149603

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Transcription factor A, mitochondrial Chains: C, G
Molecule details ›
Chains: C, G
Length: 205 amino acids
Theoretical weight: 24.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00059 (Residues: 43-245; Coverage: 83%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
DNA-directed RNA polymerase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 1128 amino acids
Theoretical weight: 127.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00411 (Residues: 105-1230; Coverage: 92%)
Gene name: POLRMT
Sequence domains:
Dimethyladenosine transferase 2, mitochondrial Chains: F, J
Molecule details ›
Chains: F, J
Length: 377 amino acids
Theoretical weight: 43.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H5Q4 (Residues: 22-396; Coverage: 95%)
Gene names: NS5ATP5, TFB2M
Sequence domains: Ribosomal RNA adenine dimethylase
Non-Template DNA Chains: D, H
Molecule details ›
Chains: D, H
Length: 50 nucleotides
Theoretical weight: 15.1 KDa
Template DNA Chains: E, I
Molecule details ›
Chains: E, I
Length: 50 nucleotides
Theoretical weight: 15.7 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 103.62Å b: 197.73Å c: 134.34Å
α: 90° β: 99.39° γ: 90°
R-values:
R R work R free
0.293 0.291 0.333
Expression system: Escherichia coli BL21(DE3)