PDBe 6e8g

Electron Microscopy
2.9Å resolution

CryoEM reconstruction of IST1-CHMP1B copolymer filament bound to ssDNA at 2.9 Angstrom resolution

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 72-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IST1 homolog Chains: A, BA, BB, C, DA, DB, E, FA, FB, G, HA, HB, I, JA, JB, K, LA, LB, M, NA, NB, O, PA, PB, Q, RA, RB, S, TA, TB, V, VA, X, XA, Z, ZA
Molecule details ›
Chains: A, BA, BB, C, DA, DB, E, FA, FB, G, HA, HB, I, JA, JB, K, LA, LB, M, NA, NB, O, PA, PB, Q, RA, RB, S, TA, TB, V, VA, X, XA, Z, ZA
Length: 366 amino acids
Theoretical weight: 40.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53990 (Residues: 1-237, 238-364; Coverage: 100%)
  • Best match: P53990-5 (Residues: 14-379)
Gene names: IST1, KIAA0174
Sequence domains: Regulator of Vps4 activity in the MVB pathway
Charged multivesicular body protein 1b Chains: AA, AB, B, CA, CB, D, EA, EB, F, GA, GB, H, IA, IB, J, KA, KB, L, MA, MB, N, OA, OB, P, QA, QB, R, SA, SB, T, UA, UB, W, WA, Y, YA
Molecule details ›
Chains: AA, AB, B, CA, CB, D, EA, EB, F, GA, GB, H, IA, IB, J, KA, KB, L, MA, MB, N, OA, OB, P, QA, QB, R, SA, SB, T, UA, UB, W, WA, Y, YA
Length: 199 amino acids
Theoretical weight: 22.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LBR1 (Residues: 1-199; Coverage: 100%)
Gene names: C18orf2, CHMP1B
Sequence domains: Snf7

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-9005
Expression system: Escherichia coli BL21(DE3)