PDBe 6e3y

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Calcitonin gene-related peptide 1 Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 3.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06881 (Residues: 83-119; Coverage: 36%)
Gene names: CALC1, CALCA
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.12 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Calcitonin gene-related peptide type 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 490 amino acids
Theoretical weight: 56.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q16602 (Residues: 22-461; Coverage: 100%)
Gene names: CALCRL, CGRPR
Sequence domains:
Receptor activity-modifying protein 1 Chain: E
Molecule details ›
Chain: E
Length: 149 amino acids
Theoretical weight: 17.05 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60894 (Residues: 27-148; Coverage: 100%)
Gene name: RAMP1
Sequence domains: Receptor activity modifying family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-8978
Expression systems:
  • Not provided
  • Escherichia coli
  • Trichoplusia ni