PDBe 6dde

Electron Microscopy
3.5Å resolution

Mu Opioid Receptor-Gi Protein Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Mu-type opioid receptor Chain: R
Molecule details ›
Chain: R
Length: 356 amino acids
Theoretical weight: 40 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 9-51, 52-358; Coverage: 88%)
  • Best match: P42866-15 (Residues: 26-290)
Gene names: Mor, Oprm, Oprm1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
DAMGO Chain: D
Molecule details ›
Chain: D
Length: 5 amino acids
Theoretical weight: 514 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-7868
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda
  • Not provided