PDBe 6d8c

Electron Microscopy
3.54Å resolution

Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 13-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Filamin-A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 306 amino acids
Theoretical weight: 34.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21333 (Residues: 1-278; Coverage: 11%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Calponin homology (CH) domain
Actin, alpha skeletal muscle Chains: H, J, K, L, M
Molecule details ›
Chains: H, J, K, L, M
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P68139 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Phalloidin Chains: N, O, P
Molecule details ›
Chains: N, O, P
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: Amanita phalloides
UniProt:
  • Canonical: P0CU65 (Residues: 10-16; Coverage: 21%)

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.54Å
Relevant EMDB volumes: EMD-7831
Expression system: Escherichia coli