PDBe 6d85

X-ray diffraction
2.2Å resolution

Structure of the Bovine p85a BH domain E217K mutant

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3-kinase regulatory subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.91 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23727 (Residues: 110-302; Coverage: 27%)
Gene name: PIK3R1
Sequence domains: RhoGAP domain

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 85.474Å b: 91.647Å c: 93.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.221
Expression system: Escherichia coli BL21(DE3)