PDBe 6crg

X-ray diffraction
2.75Å resolution

Crystal Structure of Shp2 E76K GOF Mutant in complex with SHP099

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 60.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06124 (Residues: 1-529; Coverage: 88%)
  • Best match: Q06124-2 (Residues: 1-525)
Gene names: PTP2C, PTPN11, SHPTP2
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 45.436Å b: 214.088Å c: 55.54Å
α: 90° β: 95.66° γ: 90°
R-values:
R R work R free
0.198 0.196 0.25
Expression system: Escherichia coli BL21(DE3)