PDBe 6bhd

X-ray diffraction
1.25Å resolution

Crystal structure of SETDB1 with a modified H3 peptide

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase SETDB1 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q15047 (Residues: 190-410; Coverage: 17%)
Gene names: ESET, KIAA0067, KMT1E, SETDB1
Sequence domains:
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 1.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 4-20; Coverage: 13%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 37.258Å b: 72.448Å c: 52.505Å
α: 90° β: 104.84° γ: 90°
R-values:
R R work R free
0.149 0.147 0.183
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided