PDBe 6bfc

Electron Microscopy
3.7Å resolution

Cryo-EM structure of human insulin degrading enzyme in complex with insulin

Released:

Function and Biology Details

Reaction catalysed:
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 966 amino acids
Theoretical weight: 111.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14735 (Residues: 46-1011; Coverage: 95%)
Gene name: IDE
Sequence domains:
Insulin Chains: a, b
Molecule details ›
Chains: a, b
Length: 110 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01308 (Residues: 1-110; Coverage: 100%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-7041
Expression system: Escherichia coli BL21(DE3)