PDBe 6bc1

X-ray diffraction
2.9Å resolution

A Complex between PH Domain of p190RhoGEF and Activated Rac1 Bound to a GTP Analog

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P63000 (Residues: 2-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Rho guanine nucleotide exchange factor 28 Chains: C, D
Molecule details ›
Chains: C, D
Length: 150 amino acids
Theoretical weight: 17.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q8N1W1 (Residues: 1049-1194; Coverage: 9%)
Gene names: ARHGEF28, KIAA1998, RGNEF
Sequence domains: PH domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.332Å b: 96.333Å c: 189.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.278 0.323
Expression system: Escherichia coli BL21(DE3)