PDBe 6b7o

X-ray diffraction
1.85Å resolution

Crystal structure of Legionella effector sdeD (lpg2509) H67A in complex with ADP-ribosylated Ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SdeD Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 43.37 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZSK8 (Residues: 17-397; Coverage: 96%)
Gene name: lpg2509
Ubiquitin Chains: B, C
Molecule details ›
Chains: B, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 64.677Å b: 58.768Å c: 75.111Å
α: 90° β: 114.42° γ: 90°
R-values:
R R work R free
0.161 0.159 0.201
Expression system: Escherichia coli BL21(DE3)