PDBe 6b3s

X-ray diffraction
2.8Å resolution

Crystal structure of the Fab fragment of necitumumab (Fab11F8) in complex with domain III from a cetuximab resistant variant of EGFR (sEGFRd3-S468R)

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Epidermal growth factor receptor Chains: A, B, E, I
Molecule details ›
Chains: A, B, E, I
Length: 214 amino acids
Theoretical weight: 23.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt: Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Receptor L domain
Necitumumab Fab Heavy chain Chains: C, F, H, J
Molecule details ›
Chains: C, F, H, J
Length: 222 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Necitumumab Fab Light chain Chains: D, G, K, L
Molecule details ›
Chains: D, G, K, L
Length: 213 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Mus musculus

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P2
Unit cell:
a: 151.943Å b: 80.756Å c: 172.901Å
α: 90° β: 91.5° γ: 90°
R-values:
R R work R free
0.209 0.208 0.245
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus