PDBe 6ay2

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin B Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07858 (Residues: 79-333; Coverage: 79%)
Gene names: CPSB, CTSB
Sequence domains: Papain family cysteine protease

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X1A
Spacegroup: P21
Unit cell:
a: 31.317Å b: 127.243Å c: 70.034Å
α: 90° β: 102.9° γ: 90°
R-values:
R R work R free
0.162 0.161 0.176
Expression system: Escherichia coli