PDBe 6avo

Electron Microscopy
3.8Å resolution

Cryo-EM structure of human immunoproteasome with a novel noncompetitive inhibitor that selectively inhibits activated lymphocytes

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit beta type-9 Chains: A, F
Molecule details ›
Chains: A, F
Length: 199 amino acids
Theoretical weight: 21.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28065 (Residues: 21-219; Coverage: 91%)
Gene names: LMP2, PSMB6i, PSMB9, RING12
Proteasome subunit beta type-10 Chains: B, E
Molecule details ›
Chains: B, E
Length: 234 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40306 (Residues: 40-273; Coverage: 86%)
Gene names: LMP10, MECL1, PSMB10
Sequence domains: Proteasome beta subunits C terminal
Proteasome subunit beta type-8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 204 amino acids
Theoretical weight: 22.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28062 (Residues: 73-276; Coverage: 74%)
Gene names: LMP7, PSMB5i, PSMB8, RING10, Y2
Proteasome subunit alpha type-1 Chains: G, L
Molecule details ›
Chains: G, L
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-5 Chains: H, M
Molecule details ›
Chains: H, M
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-7 Chains: I, N
Molecule details ›
Chains: I, N
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-3 Chains: J, Q
Molecule details ›
Chains: J, Q
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chains: K, R
Molecule details ›
Chains: K, R
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit alpha type-4 Chains: O, Z
Molecule details ›
Chains: O, Z
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-2 Chains: P, b
Molecule details ›
Chains: P, b
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit beta type-1 Chains: S, X
Molecule details ›
Chains: S, X
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 29-241; Coverage: 88%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: T, V
Molecule details ›
Chains: T, V
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-3 Chains: U, Y
Molecule details ›
Chains: U, Y
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: W, a
Molecule details ›
Chains: W, a
Length: 219 amino acids
Theoretical weight: 24.41 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 46-264; Coverage: 83%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-7010