PDBe 6am5

X-ray diffraction
2.39Å resolution

Crystal structure of DMF5 TCR bound to HLA-A2 presenting synthetic peptide SMLGIGIVPV

Released:
Entry authors: Riley TP, Baker BM

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
SER-MET-LEU-GLY-ILE-GLY-ILE-VAL-PRO-VAL Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: synthetic construct
Expression system: Not provided
DMF5 TCR alpha chain Chain: D
Molecule details ›
Chain: D
Length: 189 amino acids
Theoretical weight: 20.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DMF5 TCR beta chain Chain: E
Molecule details ›
Chain: E
Length: 242 amino acids
Theoretical weight: 26.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 228.106Å b: 49.9Å c: 92.455Å
α: 90° β: 95.72° γ: 90°
R-values:
R R work R free
0.203 0.201 0.244
Expression systems:
  • Escherichia coli
  • Not provided