PDBe 6ale

X-ray diffraction
2.5Å resolution

A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model

Source organisms:
Entry authors: Nam YW, Zhang M

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small conductance calcium-activated potassium channel protein 2 Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 11.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: NEW Q9H2S1 (Residues: 394-486; Coverage: 16%)
Gene name: KCNN2
Sequence domains: Calmodulin binding domain
Calmodulin-2 Chain: R
Molecule details ›
Chain: R
Length: 146 amino acids
Theoretical weight: 16.42 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: NEW P0DP30 (Residues: 5-148; Coverage: 97%)
Gene names: CaMII, Calm2, Cam2, Camb
Sequence domains: EF-hand domain pair

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: C2
Unit cell:
a: 76.653Å b: 66.913Å c: 64.712Å
α: 90° β: 92.47° γ: 90°
R R work R free
0.191 0.188 0.255
Expression system: Escherichia coli