PDBe 6a44

X-ray diffraction
2.4Å resolution

R1EN(5-227)-ubiquitin fusion

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed DNA polymerase homolog (R1); Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.54 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P0CG48 (Residues: 609-684; Coverage: 11%)
  • Canonical: NEW Q7M4J4 (Residues: 5-227; Coverage: 21%)
  • nullnull
Gene name: UBC
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P321
Unit cell:
a: 141.801Å b: 141.801Å c: 38.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.189 0.237
Expression system: Escherichia coli BL21(DE3)