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PDE-Ubiquitin

X-ray diffraction
2.182Å resolution
PDB entry 5zq3 coloured by chain, front view.
PDB entry 5zq3 coloured by chain, side view.
PDB entry 5zq3 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ubiquitin and Septation initiation protein
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Multimeric state: hetero dimer
Accessible surface area: 19535.72 Å2
Buried surface area: 2004.23 Å2
Dissociation area: 1,002.12 Å2
Dissociation energy (ΔGdiss): 0.06 kcal/mol
Dissociation entropy (TΔSdiss): 11.26 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143382
Assembly 2
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Assembly Name: Ubiquitin and Septation initiation protein
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Multimeric state: hetero dimer
Accessible surface area: 19914.21 Å2
Buried surface area: 1679.85 Å2
Dissociation area: 839.92 Å2
Dissociation energy (ΔGdiss): 0.05 kcal/mol
Dissociation entropy (TΔSdiss): 11.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143382

Macromolecules

Septation initiation protein
Chains: A, B
Length: 377 amino acids
Theoretical weight: 43.58 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6BBR6 (Residues: 240-608; Coverage: 24%)
Gene names: C3927_00465, laiD
Pfam: SidE phosphodiesterase (PDE) domain
InterPro: SidE, PDE domain
Septation initiation protein in PDB entry 5zq3, assembly 1, front view.
Septation initiation protein in PDB entry 5zq3, assembly 1, side view.
Septation initiation protein in PDB entry 5zq3, assembly 1, top view.
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Ubiquitin
Chains: C, D
Length: 78 amino acids
Theoretical weight: 8.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Pfam: Ubiquitin family
InterPro:
Ubiquitin in PDB entry 5zq3, assembly 1, front view.
Ubiquitin in PDB entry 5zq3, assembly 1, side view.
Ubiquitin in PDB entry 5zq3, assembly 1, top view.
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