PDBe 5yc7

X-ray diffraction
2Å resolution

The crystal structure of uPA in complex with 4-Bromobenzylamirne at pH7.4

Released:

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 246 amino acids
Theoretical weight: 27.72 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-424; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: R3
Unit cell:
a: 121.575Å b: 121.575Å c: 42.212Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.262
Expression system: Komagataella pastoris