5y6k › Citations

Tracking iron-associated proteomes in pathogens by a fluorescence approach.

Jiang N, Cheng T, Wang M, Chan GC, Jin L, Li H, Sun H
Metallomics 10 77-82 (2018)
Cited: 5 times
EuropePMC logo PMID: 29323384

Abstract

Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.

Reviews citing this publication (1)

  1. Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells. Jiang N, Li H, Sun H. Front Chem 7 560 (2019)

Articles citing this publication (4)

  1. Identification and Tetramer Structure of Hemin-Binding Protein SPD_0310 Linked to Iron Homeostasis and Virulence of Streptococcus pneumoniae. Cao K, Zhang T, Li N, Yang XY, Ding J, He QY, Sun X. mSystems 7 e0022122 (2022)
  2. Quantifying In Situ Structural Stabilities of Human Blood Plasma Proteins Using a Novel Iodination Protein Stability Assay. Lin HL, James I, Hyer CD, Haderlie CT, Zackrison MJ, Bateman TM, Berg M, Park JS, Daley SA, Zuniga Pina NR, Tseng YJ, Moody JD, Price JC. J Proteome Res 21 2920-2935 (2022)
  3. Cisplatin Binding to Human Serum Transferrin: A Crystallographic Study. Troisi R, Galardo F, Ferraro G, Sica F, Merlino A. Inorg Chem 62 675-678 (2023)
  4. Mitochondrial ATP synthase as a direct molecular target of chromium(III) to ameliorate hyperglycaemia stress. Wang H, Hu L, Li H, Lai YT, Wei X, Xu X, Cao Z, Cao H, Wan Q, Chang YY, Xu A, Zhou Q, Jiang G, He ML, Sun H. Nat Commun 14 1738 (2023)


Quick links