PDBe 5vu2

Electron Microscopy
6.8Å resolution

Electron cryo-microscopy of "immature" Chikungunya VLP

Released:
Primary publication:
Structural studies of Chikungunya virus maturation.
Proc. Natl. Acad. Sci. U.S.A. (2017)
PMID: 29203665
Related structures: EMD-8734

Function and Biology Details

Reaction catalysed:
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero 1440-mer (preferred)
hetero 24-mer
hetero 120-mer
hetero 144-mer
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Togavirin Chains: A, B, C, D, M, N, O, P
Molecule details ›
Chains: A, B, C, D, M, N, O, P
Length: 866 amino acids
Theoretical weight: 95.54 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A0H4CWF2 (Residues: 326-1191; Coverage: 72%)
Sequence domains: Alphavirus E2 glycoprotein
Assembly protein E3 Chains: U, V, W, X
Molecule details ›
Chains: U, V, W, X
Length: 60 amino acids
Theoretical weight: 6.93 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 266-324; Coverage: 5%)
Capsid protein Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 149 amino acids
Theoretical weight: 16.23 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 113-261; Coverage: 12%)
Spike glycoprotein E1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 46 amino acids
Theoretical weight: 4.81 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 1203-1248; Coverage: 4%)
Spike glycoprotein E2 Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 81 amino acids
Theoretical weight: 8.82 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 668-748; Coverage: 7%)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-8734
Expression system: Homo sapiens