PDBe 5um9

X-ray diffraction
2.8Å resolution

Flap endonuclease 1 (FEN1) D86N with 5'-flap substrate DNA and Sm3+

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39748 (Residues: 2-336; Coverage: 88%)
Gene names: FEN1, RAD2
Sequence domains: XPG I-region
DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.27 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3121
Unit cell:
a: 105.846Å b: 105.846Å c: 100.709Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.266
Expression systems:
  • Escherichia coli
  • Not provided