PDBe 5tsw

X-ray diffraction
2.5Å resolution

HIGH RESOLUTION CRYSTAL STRUCTURE OF A HUMAN TNF-ALPHA MUTANT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 150 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01375 (Residues: 84-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18XHF
Spacegroup: P212121
Unit cell:
a: 94.17Å b: 94.56Å c: 95.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.299
Expression system: Escherichia coli BL21(DE3)