Structure analysis

HIGH RESOLUTION CRYSTAL STRUCTURE OF A HUMAN TNF-ALPHA MUTANT

X-ray diffraction
2.5Å resolution
Source organism: Homo sapiens
Assembly composition:
homo trimer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: homo trimer
Accessible surface area: 18000 Å2
Buried surface area: 6000 Å2
Dissociation area: 3,000 Å2
Dissociation energy (ΔGdiss): 18 kcal/mol
Dissociation entropy (TΔSdiss): 25 kcal/mol
Interface energy (ΔGint): -35 kcal/mol
Symmetry number: 3
Assembly 2
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Multimeric state: homo trimer
Accessible surface area: 18200 Å2
Buried surface area: 5900 Å2
Dissociation area: 3,000 Å2
Dissociation energy (ΔGdiss): 20 kcal/mol
Dissociation entropy (TΔSdiss): 25 kcal/mol
Interface energy (ΔGint): -38 kcal/mol
Symmetry number: 3

Macromolecules

Chains: A, B, C, D, E, F
Length: 150 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01375 (Residues: 84-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Pfam: TNF(Tumour Necrosis Factor) family
InterPro:
CATH: Jelly Rolls
SCOP: TNF-like

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