› 5syg

Electron Microscopy
3.5Å resolution

Cryo-EM reconstruction of zampanolide-bound microtubule

Released:
DOI: 10.2210/pdb5syg/pdb
PDB entry 5syg coloured by chain, front view.
PDB entry 5syg coloured by chain, side view.
PDB entry 5syg coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures.
Kellogg EH, Hejab NMA, Howes S, Northcote P, Miller JH, Díaz JF, Downing KH, Nogales E
J Mol Biol 429 633-646 (2017)
PMID: 28104363
Related structures: EMD-8323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135920 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 48.68 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-437; Coverage: 97%)
Gene name: TUBA1B
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 47.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-426; Coverage: 96%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand ZPN 1 x ZPN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-8323

Quick links

Citations

17 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
16 more

4 mentions without citation

Computational Approaches to the Rational Design of Tubulin-Targeting Agents.
Pérez-Peña et al. (2023)
3 more