› 5sga

X-ray diffraction
1.8Å resolution

Structures of product and inhibitor complexes of Streptomyces griseus protease a at 1.8 Angstroms resolution. a model for serine protease catalysis

Released:
DOI: 10.2210/pdb5sga/pdb
PDB entry 5sga coloured by chain, front view.
PDB entry 5sga coloured by chain, side view.
PDB entry 5sga coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 A resolution. A model for serine protease catalysis.
James MN, Sielecki AR, Brayer GD, Delbaere LT, Bauer CA
J Mol Biol 144 43-88 (1980)
PMID: 6783761

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with specificity similar to chymotrypsin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133485 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptogrisin-A Chain: E
Molecule details ›
Chain: E
Length: 181 amino acids
Theoretical weight: 18.02 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
UniProt:
  • Canonical: P00776 (Residues: 117-297; Coverage: 70%)
Gene name: sprA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
TETRAPEPTIDE ACE-PRO-ALA-PRO-TYR Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 473 Da
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42
Unit cell:
a: 55.16Å b: 55.16Å c: 54.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

Natural protein proteinase inhibitors and their interaction with proteinases.
Bode et al. (1992)
8 more

10 mentions without citation

The limits of protein sequence comparison?
Pearson et al. (2005)
9 more