PDBe 5pnt

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A HUMAN LOW MOLECULAR WEIGHT PHOSPHOTYROSYL PHOSPHATASE. IMPLICATIONS FOR SUBSTRATE SPECIFICITY

Released:

Function and Biology Details

Reactions catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate. 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. 

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight phosphotyrosine protein phosphatase Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P24666 (Residues: 2-158; Coverage: 99%)
Gene name: ACP1
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 34.1Å b: 56.6Å c: 97.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.248
Expression system: Escherichia coli BL21