› 5of4

Electron Microscopy
4.4Å resolution

The cryo-EM structure of human TFIIH

Released:
DOI: 10.2210/pdb5of4/pdb
PDB entry 5of4 coloured by chain, front view.
PDB entry 5of4 coloured by chain, side view.
PDB entry 5of4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cryo-electron microscopy structure of human transcription factor IIH.
Greber BJ, Nguyen THD, Fang J, Afonine PV, Adams PD, Nogales E
Nature 549 414-417 (2017)
PMID: 28902838
Related structures: EMD-3802

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-148279 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 62.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19447 (Residues: 265-782; Coverage: 66%)
Gene names: ERCC3, XPB, XPBC
Sequence domains:
General transcription and DNA repair factor IIH helicase subunit XPD Chain: B
Molecule details ›
Chain: B
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18074 (Residues: 1-760; Coverage: 100%)
Gene names: ERCC2, XPD, XPDC
Sequence domains:
General transcription factor IIH subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q92759 (Residues: 383-462; Coverage: 17%)
Gene name: GTF2H4
Sequence domains: Transcription factor Tfb2 (p52) C-terminal domain
General transcription factor IIH subunit 2 Chain: E
Molecule details ›
Chain: E
Length: 395 amino acids
Theoretical weight: 44.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13888 (Residues: 1-395; Coverage: 100%)
Gene names: BTF2P44, GTF2H2
Sequence domains:
General transcription factor IIH subunit 3 Chain: F
Molecule details ›
Chain: F
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13889 (Residues: 1-308; Coverage: 100%)
Gene name: GTF2H3
Sequence domains: Transcription factor Tfb4
General transcription factor IIH subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Gene names: C6orf175, GTF2H5, TTDA
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
MAT1 Chain: H
Molecule details ›
Chain: H
Length: 124 amino acids
Theoretical weight: 10.57 KDa
Source organism: Homo sapiens
Unassigned secondary structure elements. Chain: Z
Molecule details ›
Chain: Z
Length: 270 amino acids
Theoretical weight: 23 KDa
Source organism: Homo sapiens
Unassigned secondary structure elements (p52 region) Chain: Y
Molecule details ›
Chain: Y
Length: 232 amino acids
Theoretical weight: 19.76 KDa
Source organism: Homo sapiens
Unassigned secondary structure elements (XPB NTE region) Chain: X
Molecule details ›
Chain: X
Length: 78 amino acids
Theoretical weight: 6.66 KDa
Source organism: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-3802

Quick links

Citations

16 review citations

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
15 more

12 mentions without citation

The essential and multifunctional TFIIH complex.
Rimel et al. (2018)
11 more