PDBe 5lnp

X-ray diffraction
1.99Å resolution

Domain-swapped dimer of human Dishevelled2 DEP domain: monoclinic crystal form crystallised from monomeric fraction

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Segment polarity protein dishevelled homolog DVL-2 Chains: A, C, D
Molecule details ›
Chains: A, C, D
Length: 97 amino acids
Theoretical weight: 10.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14641 (Residues: 416-510; Coverage: 13%)
Gene name: DVL2
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
Segment polarity protein dishevelled homolog DVL-2 Chain: B
Molecule details ›
Chain: B
Length: 97 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14641 (Residues: 416-510; Coverage: 13%)
Gene name: DVL2
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 61.583Å b: 60.181Å c: 66.292Å
α: 90° β: 117.71° γ: 90°
R-values:
R R work R free
0.196 0.194 0.246
Expression system: Escherichia coli BL21(DE3)