PDBe 5l4q

X-ray diffraction
1.97Å resolution

Crystal Structure of Adaptor Protein 2 Associated Kinase 1 (AAK1) in Complex with LKB1 (AAK1 Dual Inhibitor)

Released:
Source organism: Homo sapiens
Entry authors: Sorrell FJ, Williams E, Fox N, Abdul Azeez KR, Gileadi O, von Delft F, Edwards AM, Bountra C, Elkins JM, Knapp S

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AP2-associated protein kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 38.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2M2I8 (Residues: 27-365; Coverage: 35%)
Gene names: AAK1, KIAA1048
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 61.8Å b: 55.05Å c: 86.57Å
α: 90° β: 104.44° γ: 90°
R-values:
R R work R free
0.208 0.207 0.226
Expression system: Escherichia coli BL21(DE3)