› 5kqv

X-ray diffraction
4.4Å resolution

Insulin receptor ectodomain construct comprising domains L1,CR,L2, FnIII-1 and alphaCT peptide in complex with bovine insulin and FAB 83-14 (REVISED STRUCTURE)

Released:
DOI: 10.2210/pdb5kqv/pdb
PDB entry 5kqv coloured by chain, front view.
PDB entry 5kqv coloured by chain, side view.
PDB entry 5kqv coloured by chain, top view.
Source organisms:
Primary publication:
How insulin engages its primary binding site on the insulin receptor.
Menting JG, Whittaker J, Margetts MB, Whittaker LJ, Kong GK, Smith BJ, Watson CJ, Záková L, Kletvíková E, Jiráček J, Chan SJ, Steiner DF, Dodson GG, Brzozowski AM, Weiss MA, Ward CW, Lawrence MC
Nature 493 241-5 (2013)
PMID: 23302862

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-230723 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
Insulin A chain Chains: A, I
Molecule details ›
Chains: A, I
Length: 21 amino acids
Theoretical weight: 2.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01317 (Residues: 85-105; Coverage: 26%)
Gene name: INS
Insulin B chain Chains: B, J
Molecule details ›
Chains: B, J
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01317 (Residues: 25-54; Coverage: 37%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
MONOCLONAL ANTIBODY FAB 83-14 - HEAVY CHAIN Chains: C, P
Molecule details ›
Chains: C, P
Length: 220 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
MONOCLONAL ANTIBODY FAB 83-14 - LIGHT CHAIN Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 214 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Insulin receptor subunit alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 609 amino acids
Theoretical weight: 69.98 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-620; Coverage: 44%)
Gene name: INSR
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 118.15Å b: 140.1Å c: 190.022Å
α: 90° β: 95.04° γ: 90°
R-values:
R R work R free
0.231 0.229 0.266
Expression system: Cricetulus griseus

Quick links

Citations

59 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
58 more

1 mention without citation

Functional selectivity of insulin receptor revealed by aptamer-trapped receptor structures.
Kim et al. (2022)