› 5khj

X-ray diffraction
2.01Å resolution

HCN2 CNBD in complex with uridine-3', 5'-cyclic monophosphate (cUMP)

Released:
DOI: 10.2210/pdb5khj/pdb
PDB entry 5khj coloured by chain, front view.
PDB entry 5khj coloured by chain, side view.
PDB entry 5khj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cyclic Purine and Pyrimidine Nucleotides Bind to the HCN2 Ion Channel and Variably Promote C-Terminal Domain Interactions and Opening.
Ng LCT, Putrenko I, Baronas V, Van Petegem F, Accili EA
Structure 24 1629-1642 (2016)
PMID: 27568927

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88703 (Residues: 443-643; Coverage: 23%)
Gene names: Bcng2, Hac1, Hcn2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 6SY 2 x 6SY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 46.486Å b: 89.925Å c: 98.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels: from Biophysics to Pharmacology of a Unique Family of Ion Channels.
Sartiani et al. (2017)
2 more