› 5k4p

X-ray diffraction
1.32Å resolution

Catalytic Domain of MCR-1 phosphoethanolamine transferase

Released:
DOI: 10.2210/pdb5k4p/pdb
PDB entry 5k4p coloured by chain, front view.
PDB entry 5k4p coloured by chain, side view.
PDB entry 5k4p coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Structure of the catalytic domain of the colistin resistance enzyme MCR-1.
Stojanoski V, Sankaran B, Prasad BV, Poirel L, Nordmann P, Palzkill T
BMC Biol 14 81 (2016)
PMID: 27655155

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable phosphatidylethanolamine transferase Mcr-1 Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 38.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R6L508 (Residues: 214-541; Coverage: 61%)
Gene names: APZ14_31440, mcr-1, mcr1
Sequence domains: Sulfatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

2 bound ligands:
Ligand ZN 10 x ZN
Ligand SOR 1 x SOR
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 59.08Å b: 59.08Å c: 186.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.178

Quick links

Citations

9 review citations

Polymyxins: Antibacterial Activity, Susceptibility Testing, and Resistance Mechanisms Encoded by Plasmids or Chromosomes.
Poirel et al. (2017)
8 more

8 mentions without citation

Recent progress on elucidating the molecular mechanism of plasmid-mediated colistin resistance and drug design.
Kai et al. (2020)
7 more