› 5k1r

X-ray diffraction
2.1Å resolution

Structure of Burkholderia pseudomallei K96243 sphingosine-1-phosphate lyase Bpss2021

Released:
DOI: 10.2210/pdb5k1r/pdb
PDB entry 5k1r coloured by chain, front view.
PDB entry 5k1r coloured by chain, side view.
PDB entry 5k1r coloured by chain, top view.
Source organism: Burkholderia pseudomallei K96243
Primary publication:
Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei.
McLean CJ, Marles-Wright J, Custodio R, Lowther J, Kennedy AJ, Pollock J, Clarke DJ, Brown AR, Campopiano DJ
J Lipid Res 58 137-150 (2017)
PMID: 27784725

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 53.3 KDa
Source organism: Burkholderia pseudomallei K96243
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63IP8 (Residues: 21-493; Coverage: 96%)
Gene name: BPSS2021
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments

1 bound ligand:
Ligand PLP 2 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 59.6Å b: 126.776Å c: 59.725Å
α: 90° β: 97.51° γ: 90°
R-values:
R R work R free
0.176 0.174 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Macrophage-Bacteria Interactions-A Lipid-Centric Relationship.
Teng et al. (2017)
2 more

4 mentions without citation

Sphingolipid biosynthesis in man and microbes.
Harrison et al. (2018)
3 more