› 5jqw

X-ray diffraction
2.06Å resolution

The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4 in complex with ADP

Released:
DOI: 10.2210/pdb5jqw/pdb
PDB entry 5jqw coloured by chain, front view.
PDB entry 5jqw coloured by chain, side view.
PDB entry 5jqw coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Tan K, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthase Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 40.87 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NGE8 (Residues: 1-365; Coverage: 100%)
Gene names: FTT_0897, purK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 85.23Å b: 85.23Å c: 103.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.229
Expression system: Escherichia coli

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