› 5jji

X-ray diffraction
2.6Å resolution

Rho transcription termination factor bound to rU7 and 6 ADP-BeF3 molecules

Released:
DOI: 10.2210/pdb5jji/pdb
PDB entry 5jji coloured by chain, front view.
PDB entry 5jji coloured by chain, side view.
PDB entry 5jji coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor.
Thomsen ND, Lawson MR, Witkowsky LB, Qu S, Berger JM
Proc Natl Acad Sci U S A 113 E7691-E7700 (2016)
PMID: 27856760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142787 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination factor Rho Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 420 amino acids
Theoretical weight: 47.95 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG30 (Residues: 2-417; Coverage: 99%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Structure domains:
rU12: 5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)- 3' Chain: G
Molecule details ›
Chain: G
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 6 x MG
Ligand BEF 6 x BEF
Ligand MPD 1 x MPD
1 modified residue:
Ligand MSE 102 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 68.997Å b: 198.567Å c: 111.448Å
α: 90° β: 104.36° γ: 90°
R-values:
R R work R free
0.22 0.219 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Rho Protein: Roles and Mechanisms.
Mitra et al. (2017)
5 more

8 mentions without citation

Rho-dependent transcription termination: a revisionist view.
Hao et al. (2021)
7 more