› 5jej

X-ray diffraction
2Å resolution

Phosphorylated STING in complex with IRF-3 CTD

Released:
DOI: 10.2210/pdb5jej/pdb
PDB entry 5jej coloured by chain, front view.
PDB entry 5jej coloured by chain, side view.
PDB entry 5jej coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for concerted recruitment and activation of IRF-3 by innate immune adaptor proteins.
Zhao B, Shu C, Gao X, Sankaran B, Du F, Shelton CL, Herr AB, Ji JY, Li P
Proc Natl Acad Sci U S A 113 E3403-12 (2016)
PMID: 27302953

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172055 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stimulator of interferon genes protein Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 39 amino acids
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86WV6 (Residues: 342-379; Coverage: 10%)
Gene names: ERIS, MITA, STING, STING1, TMEM173
Interferon regulatory factor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14653 (Residues: 189-427; Coverage: 56%)
Gene name: IRF3
Sequence domains: Interferon-regulatory factor 3
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 3 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P2
Unit cell:
a: 64.076Å b: 56.401Å c: 75.548Å
α: 90° β: 104.64° γ: 90°
R-values:
R R work R free
0.18 0.176 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

28 review citations

cGAS-cGAMP-STING: The three musketeers of cytosolic DNA sensing and signaling.
Tao et al. (2016)
27 more

6 mentions without citation

STING modulators: Predictive significance in drug discovery.
Cui et al. (2019)
5 more