› 5j99

X-ray diffraction
1.7Å resolution

Ambient temperature transition state structure of arginine kinase - crystal 8/Form I

Released:
DOI: 10.2210/pdb5j99/pdb
PDB entry 5j99 coloured by chain, front view.
PDB entry 5j99 coloured by chain, side view.
PDB entry 5j99 coloured by chain, top view.
Source organism: Limulus polyphemus
Primary publication:
The Sampling of Conformational Dynamics in Ambient-Temperature Crystal Structures of Arginine Kinase.
Godsey MH, Davulcu O, Nix JC, Skalicky JJ, Brüschweiler RP, Chapman MS
Structure 24 1658-1667 (2016)
PMID: 27594681

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine kinase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.25 KDa
Source organism: Limulus polyphemus
Expression system: Escherichia coli
UniProt:
  • Canonical: P51541 (Residues: 1-357; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand ARG 1 x ARG
Ligand NO3 1 x NO3
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 63.871Å b: 66.035Å c: 86.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.183
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural and dynamical description of the enzymatic reaction of a phosphohexomutase.
Stiers et al. (2019)
1 more

2 mentions without citation

The Sampling of Conformational Dynamics in Ambient-Temperature Crystal Structures of Arginine Kinase.
Godsey et al. (2016)
1 more