› 5izz

X-ray diffraction
1.6Å resolution

Crystal structure of a marine metagenome TRAP solute binding protein specific for aromatic acid ligands (Sorcerer II Global Ocean Sampling Expedition, unidentified microbe, locus tag GOS_1523157, Triple Surface Mutant K158A_K223A_K313A) in complex with metahydroxyphenylacetate, thermal exchange of ligand

Released:
DOI: 10.2210/pdb5izz/pdb
PDB entry 5izz coloured by chain, front view.
PDB entry 5izz coloured by chain, side view.
PDB entry 5izz coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Vetting MW, Al Obaidi NF, Hogle SL, Dupont CL, Almo SC

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP TRANSPORTER SOLUTE BINDING PROTEIN Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 36.83 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand 3HP 1 x 3HP
Ligand PEG 5 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.784Å b: 84.275Å c: 45.91Å
α: 90° β: 113.48° γ: 90°
R-values:
R R work R free
0.117 0.115 0.153
Expression system: Escherichia coli BL21(DE3)

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