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Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon

X-ray diffraction
1.41Å resolution
PDB entry 5hw5 coloured by chain, front view.
PDB entry 5hw5 coloured by chain, side view.
PDB entry 5hw5 coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Beta-lactamase TEM
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Multimeric state: monomeric
Accessible surface area: 10532.44 Å2
Buried surface area: 516.45 Å2
Dissociation area: 83.39 Å2
Dissociation energy (ΔGdiss): -1.58 kcal/mol
Dissociation entropy (TΔSdiss): 1.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-158652
Assembly 2
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Assembly Name: Beta-lactamase TEM
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Multimeric state: monomeric
Accessible surface area: 10770.37 Å2
Buried surface area: 670.36 Å2
Dissociation area: 83.1 Å2
Dissociation energy (ΔGdiss): -1.58 kcal/mol
Dissociation entropy (TΔSdiss): 1.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-158652
Assembly 3
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Assembly Name: Beta-lactamase TEM
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Multimeric state: monomeric
Accessible surface area: 10644.4 Å2
Buried surface area: 517.17 Å2
Dissociation area: 83.29 Å2
Dissociation energy (ΔGdiss): -1.57 kcal/mol
Dissociation entropy (TΔSdiss): 1.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-158652
Assembly 4
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Assembly Name: Beta-lactamase TEM
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Multimeric state: monomeric
Accessible surface area: 10654.75 Å2
Buried surface area: 517.13 Å2
Dissociation area: 84.11 Å2
Dissociation energy (ΔGdiss): -1.57 kcal/mol
Dissociation entropy (TΔSdiss): 1.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-158652

Macromolecules

Beta-lactamase TEM
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 28.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P62593 (Residues: 24-286; Coverage: 100%)
Gene names: bla, blaT-3, blaT-4, blaT-5, blaT-6
Pfam:
InterPro:
CATH: DD-peptidase/beta-lactamase superfamily
Beta-lactamase TEM in PDB entry 5hw5, assembly 1, front view.
Beta-lactamase TEM in PDB entry 5hw5, assembly 1, side view.
Beta-lactamase TEM in PDB entry 5hw5, assembly 1, top view.
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