Function and Biology

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: NEDD4L and UbV NL.1

Source organism: Homo sapiens
Biochemical function: ubiquitin-protein transferase activity
Biological process: not assigned
Cellular component: not assigned

EC 2.3.2.26: HECT-type E3 ubiquitin transferase

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Systematic name:
S-ubiquitinyl-[HECT-type E3-ubiquitin transferase]-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming)
Alternative Name(s):
  • HECT E3 ligase
  • Ubiquitin transferase HECT-E3

EC 2.3.2.36: RING-type E3 ubiquitin transferase (cysteine targeting)

Reaction catalysed:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Systematic name:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (thioester bond-froming; RING-type)
Alternative Name(s):
  • RING E3 ligase

GO terms

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF00240
Domain description: Ubiquitin family
Occurring in:
  1. Ubiquitin
The deposited structure of PDB entry 5hpk contains 1 copy of Pfam domain PF00240 (Ubiquitin family) in Ubiquitin. Showing 1 copy in chain B.

PF00632
Domain description: HECT-domain (ubiquitin-transferase)
Occurring in:
  1. E3 ubiquitin-protein ligase NEDD4-like
The deposited structure of PDB entry 5hpk contains 1 copy of Pfam domain PF00632 (HECT-domain (ubiquitin-transferase)) in E3 ubiquitin-protein ligase NEDD4-like. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR000569
Domain description: HECT domain
Occurring in:
  1. E3 ubiquitin-protein ligase NEDD4-like
IPR019956
Domain description: Ubiquitin domain
Occurring in:
  1. Ubiquitin
IPR000626
Domain description: Ubiquitin-like domain
Occurring in:
  1. Ubiquitin
IPR019954
Domain description: Ubiquitin conserved site
Occurring in:
  1. Ubiquitin
IPR029071
Domain description: Ubiquitin-like domain superfamily
Occurring in:
  1. Ubiquitin
IPR035983
Domain description: HECT, E3 ligase catalytic domain
Occurring in:
  1. E3 ubiquitin-protein ligase NEDD4-like