PDBe 5hjd

X-ray diffraction
2.81Å resolution

AF9 YEATS in complex with histone H3 Crotonylation at K18

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein AF-9 Chains: A, C, E, G, K, N, Q, T
Molecule details ›
Chains: A, C, E, G, K, N, Q, T
Length: 140 amino acids
Theoretical weight: 16.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42568 (Residues: 1-138; Coverage: 24%)
Gene names: AF9, MLLT3, YEATS3
Sequence domains: YEATS family
Structure domains: Immunoglobulin-like
Histone H3.1 Chains: B, D, F, H, I, J, L, M
Molecule details ›
Chains: B, D, F, H, I, J, L, M
Length: 7 amino acids
Theoretical weight: 910 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 15-21; Coverage: 5%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 91.264Å b: 101.573Å c: 102.076Å
α: 90° β: 103.06° γ: 90°
R-values:
R R work R free
0.243 0.242 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided